How to grow your cable bacteria?

Keywords: Protocols, cable bacteria

USA

Protocol for using autoclaved intertidal sediment as a medium to enrich marine cable bacteria

Full article can be found at: https://doi.org/10.1016/j.xpro.2022.101604

Cheng Li, Clare E. Reimers, Peter J. Chace

Corresponding author:

C. Li

Cable bacteria (CB) are non-isolated filamentous bacteria in the family of Desulfobulbaceae, known for fostering centimeter-long electron transfer in sediments with pronounced redox zonation. This protocol details steps to extract CB filaments from cultured natural sediment, inoculate autoclaved sediment with extracted filaments, and subsequently evaluate the growth and enrichment of CB. We also describe the approaches for collecting suitable sediment, preparing autoclaved sediment, and manufacturing glass needles and hooks to extract CB.

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